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目的 研究血管生成抑制因子———内皮抑素 (endostatin)对结肠癌肝转移的影响。 方法 32只裸鼠用脾脏切除法建立人结肠癌裸鼠肝转移模型 ,然后分为 4组 ,每组 8只。分别自腹腔注射生理盐水 (对照组 )、5 氟脲嘧啶 (5 Fu) (治疗Ⅰ组 )、endostatin(治疗Ⅱ组 )以及 5 Fu与endostatin二药联合 (治疗Ⅲ组 ) ,比较各组肝转移率。 结果 对照组、治疗Ⅰ组、治疗Ⅱ组和治疗Ⅲ组肿瘤肝转移率分别为 10 0 %、75 %、2 5 %和 2 5 %。治疗Ⅱ组和治疗Ⅲ组肝转移率低于对照组及治疗Ⅰ组 ,差异有显著性意义 (与对照组比较P <0 0 1;与治疗Ⅰ组比较P <0 0 5 )。endostatin及endostatin与 5 Fu联合应用使肿瘤肝转移率明显降低 ,平均肝转移瘤数下降 ,动物的生存期延长。 结论 endostatin可有效地干预结肠癌肝转移的形成 ,较 5 Fu有更大的临床潜在应用价值。
Objective To study the effect of endostatin, an angiogenesis inhibitor, on hepatic metastasis of colon cancer. Methods Thirty-two nude mice were used to establish a liver metastasis model of human colon cancer in nude mice by splenectomy. Then they were divided into 4 groups, 8 in each group. Intraperitoneal injection of saline (control group), 5-fluorouracil (5 Fu) (treatment I group), endostatin (treatment II group), and 5 Fu combined with endostatin agent (treatment group III) were used to compare liver metastases in each group. rate. Results The liver metastasis rates in the control, treatment I, treatment II, and treatment III groups were 100%, 75%, 25%, and 25%, respectively. The rate of liver metastasis in treatment group II and treatment group III was lower than that in control group and treatment group I. The difference was significant (P <0 1 compared with control group; P <0 05 compared with treatment group I). The combination of endostatin and endostatin with 5 Fu significantly reduced the rate of hepatic metastasis, decreased the average number of hepatic metastases, and prolonged the survival of animals. Conclusion Endostatin can effectively interfere with the formation of hepatic metastasis of colon cancer, and has greater potential clinical application value than 5 Fu.