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地塞米松是一种治疗多种变态反应性疾病和炎症的重要药物。体液中地塞米松的含量测定通常采用放射免疫测定法,但该法费时且需要昂贵的特异性抗体。此外,尚有用正相或反相高效液相色谱测定人体血浆地塞米松含量的方法,这些方法虽然专一、灵敏,但分析前一般需要2～5步提取程序,仍然存在繁琐、费时等问题。本文介绍一种在固相提取柱上提取血浆中地塞米松,通过反相高效液相色谱紫外检测器定量的方法。操作条件:高压液相色谱仪配有210型样品注射阀,Beckman-160型吸光度检测
Dexamethasone is an important drug for the treatment of a variety of allergic diseases and inflammations. Determination of dexamethasone in bodily fluids is usually radioimmunoassay, but this method is time-consuming and requires expensive specific antibodies. In addition, there are still normal phase or reversed-phase high performance liquid chromatography determination of human plasma dexamethasone content, although these methods are specific and sensitive, but generally require 2 to 5 extraction procedures before analysis, there are still cumbersome, time-consuming and other issues . This article describes a method for the extraction of plasma dexamethasone on a solid phase extraction column by reversed-phase high performance liquid chromatography with a UV detector. Operating Conditions: High Pressure Liquid Chromatograph with Model 210 Injection Valve, Beckman-160 Absorbance Detection