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选用初重为(39.68±3.05)g的草鱼(Ctenopharyngodon idellus)幼鱼作为试验对象,在全植物蛋白基础饲料中添加植酸酶,设5种不同的植酸酶添加水平(500 U/kg、750 U/kg、1 000 U/kg、1 250 U/kg、2 500 U/kg),另设2个对照组,对照组1:全植物蛋白基础饲料中只添加1.5%的无机磷;对照组2:全植物蛋白基础饲料不添加植酸酶和无机磷,通过60 d的试验,探讨和研究植酸酶对草鱼生长、体组成及各组织磷含量的影响。结果表明,对照组1鱼体的特定生长率最大,饲料系数显著低于其它各组(P<0.05);与对照组2相比较,全植物蛋白饲料中添加植酸酶后,当植酸酶添加水平大于1 000 U/kg时,草鱼的饲料系数显著降低、蛋白质效率得到显著提高;植酸酶对草鱼的形体指标没有显著影响;当植酸酶添加水平大于1 000 U/kg时鱼体粗脂肪含量有所降低;当植酸酶添加水平为1 000 U/kg时,其肌肉粗脂肪含量显著提高;添加植酸酶后鱼体、肌肉和脊椎骨中的磷含量有一定提高,且当植酸酶添加水平为1 250 U/kg时,肌肉和脊椎骨中的磷含量显著提高。结果表明,当植酸酶添加水平在1 000 U/kg～1250 U/kg时,与对照组2相比较,全植物蛋白饲料中添加植酸酶可提高草鱼的特定生长率和蛋白质效率、降低饲料系数,并显著提高草鱼磷的积累。若欲减少无机磷添加量,建议植酸酶添加水平为1 000 U/kg～1 250 U/kg。
The juvenile fish of Ctenopharyngodon idellus with the initial weight of (39.68 ± 3.05) g were used as the experimental subjects. Phytase was added into the whole plant protein-based feed and five different levels of phytase (500 U / kg, 750 U / kg, 1 000 U / kg, 1 250 U / kg and 2 500 U / kg respectively), and another two control groups. In control group 1, only 1.5% inorganic phosphorus was added to the whole plant protein diet; 2: The phytase and inorganic phosphorus were not added to the basal diet of whole plant protein, and the effects of phytase on the growth, body composition and phosphorus content of grass carp were studied and studied through 60 days. The results showed that the specific growth rate of fish in control group 1 was the highest and the feed coefficient was significantly lower than that in other groups (P <0.05). Compared with control group 2, phytase Feed additive of grass carp decreased significantly and protein efficiency increased significantly when the level of addition was over 1 000 U / kg. Phytase had no significant effect on the body index of grass carp. When phytase was added above 1 000 U / kg, The content of crude fat decreased; when the level of phytase added was 1 000 U / kg, the content of crude fat in muscle increased significantly; Phosphorus content in fish, muscle and vertebra increased slightly after adding phytase, At phytase levels of 1,250 U / kg, phosphorus levels in muscles and vertebrae were significantly increased. The results showed that adding phytase to the whole plant protein feed increased the specific growth rate and protein efficiency of grass carp and decreased the phytase level when the phytase level was between 1 000 U / kg and 1250 U / kg Feed coefficient, and significantly increase the phosphorus accumulation in grass carp. To reduce the amount of inorganic phosphorus added, it is recommended to add phytase levels of 1 000 U / kg ~ 1 250 U / kg.