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全血胆碱酯酶的活性测定有三氯化铁比色法。BTB比色法,Asch—DTNB分光光度法。基层实验室以三氯化铁比色法用得较多,按劳动医学1987,四卷第一期专辑推荐的测定方法,我们进行了140份全血胆碱酯酶的活性测定,在测定过程中,本实验发现在样品测定时,“于A管(样品管)和B管(对照管)中各加入0.98ml水,加入20μl末梢血……”操作步骤中,0.98ml水应改用0.98ml磷酸缓冲液为宜。经本实验多次实验证明:若A、B管加入0.98ml水,使酶活性值偏
Whole blood cholinesterase activity assay ferric chloride colorimetric method. BTB colorimetric method, Asch-DTNB spectrophotometry. Grassroots laboratories to use more ferric chloride colorimetric method, according to Labor Medicine 1987, four volumes of the first album recommended measurement method, we conducted 140 whole blood cholinesterase activity in the determination of the process , The experiment found that in the determination of the sample, “in the A tube (tube) and B tube (control tube) were added 0.98ml water, adding 20μl of peripheral blood ... ...” steps, 0.98ml water should be used 0.98ml ml phosphate buffer is appropriate. After experiments in this experiment proved that: If A, B tube by adding 0.98ml water, the enzyme activity value partial