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背景:力学应变在骨重建中起重要作用。然而,力学应变是否影响破骨细胞的凋亡仍不清楚。目的:观察力学应变对体外培养破骨细胞凋亡的影响。方法:对小鼠单核细胞RAW264.7采用巨噬细胞集落刺激因子和破骨细胞分化因子诱导,抗酒石酸酸性磷酸酶染色和骨吸收实验确定成功诱导出了破骨细胞。实验共分为3组,对诱导的破骨细胞分别施加0(对照组),2500和5000με的基底拉伸应变3d,1h/次,1次/d。结果与结论:与对照组相比,生理强度载荷2500με阻止了破骨细胞的凋亡和线粒体膜电位的下降。但是,病理强度载荷5000με对破骨细胞的凋亡和线粒体膜电位没有影响。
Background: Mechanical strain plays an important role in bone remodeling. However, whether mechanical strain affects osteoclast apoptosis remains unclear. Objective: To observe the effect of mechanical strain on the apoptosis of cultured osteoclasts in vitro. Methods: The osteoclasts were successfully induced in RAW264.7 cells induced by macrophage colony-stimulating factor and osteoclast differentiation factor, tartrate-resistant acid phosphatase staining and bone resorption assay. The experiment was divided into 3 groups, 0 (control group) was induced on the induced osteoclasts, and the tensile strain at the base of 2500 and 5000με was 3d, 1h / time, 1 time / d. RESULTS AND CONCLUSION: Compared with the control group, the physiological strength load 2500με prevented the apoptosis of osteoclasts and the decrease of mitochondrial membrane potential. However, a pathological intensity load of 5000με had no effect on osteoclast apoptosis and mitochondrial membrane potential.