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目的观察酪酸梭菌对葡聚糖硫酸钠(DSS)诱导的实验性溃疡性结肠炎(UC)小鼠结肠粘膜中ITF、NF-κB和TNF-α表达的影响,了解酪酸梭菌治疗UC的效果及可能的作用机制。方法用3%的DSS建立小鼠溃疡性结肠炎的模型。60只BALB/c小鼠随机分为正常组、模型(空插灌胃针)组、阴性对照(生理盐水)组、阳性对照(美沙拉嗪)组、酪酸梭菌低剂量(106CFU/ml)组、高剂量(107CFU/ml)组。观察小鼠疾病活动指数(DAI)评分和肠道病理形态改变,以检测各处理组的效果;免疫组化法检测结肠组织中ITF和NF-κB的表达;放射免疫法检测TNF-α的表达。结果酪酸梭菌可以影响急性期UC小鼠的一般情况,降低DAI积分,减轻肠道的组织学损伤程度;与模型组和阴性对照组相比,结肠中ITF的表达升高(P<0.05);NF-κB和TNF-α的表达下降(P<0.05),其中低剂量组和阳性对照组效果相当(P>0.05)。结论酪酸梭菌对急性UC有治疗作用,疗效与剂量有一定的相关性,其部分机制可能与酪酸梭菌增加ITF的表达,抑制NF-κB和TNF-α的表达有关。
Objective To investigate the effects of Clostridium butyricum on the expression of ITF, NF-κB and TNF-α in colonic mucosa induced by dextran sodium sulfate (DSS) in mice with experimental ulcerative colitis (UC) Effect and possible mechanism of action. Methods A mouse model of ulcerative colitis was established with 3% DSS. Sixty BALB / c mice were randomly divided into normal group, model group (empty perfusion and gastric perfusion group), negative control group (normal saline group), positive control group (mesalazine) group and low Clostridium butyricum (106CFU / Group, a high dose (107 CFU / ml) group. The disease activity index (DAI) scores of mice and pathological changes of intestinal tract were observed to detect the effect of each treatment group; the expression of ITF and NF-κB in colonic tissue was detected by immunohistochemistry; the expression of TNF-α was detected by radioimmunoassay . Results Clostridium butyricum could affect the general situation of acute UC mice, reduce the DAI score and reduce the intestinal histological damage. Compared with the model group and negative control group, Clostridium butyricum increased ITF expression (P <0.05) ; The expression of NF-κB and TNF-α decreased (P <0.05), and the effect of low dose group and positive control group was similar (P> 0.05). Conclusion Clostridium butyricum has a therapeutic effect on acute UC. The curative effect is dose-dependent. Some mechanisms may be related to Clostridium butyricum which increases the expression of ITF and inhibits the expression of NF-κB and TNF-α.