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含抗透明带抗体各种方法检测血清ＡＳＡｂ阳性率分别为：ＴＳＡＴ３６．４％～５２．７％、ＧＡＴ４％～２４．３％ＳＩＴ１．５％～１４％、ＴＡＴ１５．６％～２１％ＥＬＩＳＡ１２．５％～４０％、固相酶、免疫斑点法（ＩＤＴ、免疫洗选法２３．２％～２７．１％、ＩＢＴ９％～２１％。其中ＴＳＡＴ结果较高，主要干扰可能来自ＡＢＯ血型抗原和一种血清因子，即β－脂蛋白－甾体轭合物。前者吸附于精子表面可与血清中天然抗体发生反应，后者在妇女月经周期的黄体期、妊娠期及口服避孕药时可增高，引起精子凝集。ＳＩＴ结果较低与该法仅能测出补体依赖性抗体有关，由于只有精子特异性抗原才能使精子制动素升高，因而该试验特异地反映了精子制动抗体的水平。目前普遍应用的固相酶、ＥＬＩＳＡ、ＩＤＴ、ＩＢＴ等法结果相近，操作方便，有利于临床应用比较。ＢＡ－ＥＬＩＳＡ和ＡＢＣ－ＥＬＩＳＡ敏感性高于传统的精子凝集及制动试验，是常规ＥＬＩＳＡ的４～８倍［４，５］因而是潜在的定量观察ＡｓＡｂ的较好方法。ＷＨＯ介绍的ＭＡＲ、ＩＢＴ两法是检测精子结合抗体的简单易行的方法２其中尤以ＭＡＲ值得在基层医院推广应用，该法经改良试剂易得，操作简便，诊断明确，可检测精子表面ＩｇＧ类ＡｓＡ?
The positive rates of serum ASAb detected by various methods including anti-zona pellucida antibody were: TSAT 36.4% -52.7%, GAT4% -24.3% SIT 1.5% -14%, TAT 15.6% -21% ELISA12. 5% ~ 40%, enzyme-linked immunosorbent assay (IDT), immune wash method 23.2% ~ 27.1%, IBT9% ~ 21% .The TSAT results are higher, the main interference may come from ABO blood group antigen and A serum factor, the beta-lipoprotein-steroid conjugate, which adsorbs to the surface of sperm and reacts with natural antibodies in the serum, which is elevated during the luteal phase of women’s menstrual cycle, during pregnancy and when taking oral contraceptives , Resulting in sperm agglutination.SIT lower results with the method can only detect complement-dependent antibodies, because only sperm-specific antigen can make sperm arrestin elevated, so the test specifically reflects the level of sperm brake antibody At present, the commonly used methods such as enzyme, ELISA, IDT, IBT and so on The sensitivity of BA-ELISA and ABC-ELISA is higher than that of traditional sperm agglutination and immobilization test, which is 4 to 8 times higher than that of conventional ELISA [4, 5] and therefore is a potential quantitative observation AsAb the better way.WHO introduction of MAR, IBT two methods is to detect sperm antibodies in combination with simple and easy method 2 which is worth MAR in the grass-roots hospitals to promote the application of the improved reagent easy to get the method, easy to operate, the diagnosis is clear , Can detect sperm surface IgG class AsA?