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【目的】阐明大气CO2浓度升高对外来入侵昆虫西花蓟马Frankliniella occidentalis及其本地近缘种花蓟马F.intonsa的影响机制。【方法】测定和分析了CO2人工气候箱内不同CO2浓度(400μL/L和800μL/L)下饲养3代的这两种蓟马体内3种解毒酶[羧酸酯酶(CarE)、乙酰胆碱酯酶(AchE)和微粒体多功能氧化酶(MFO)]和3种保护酶[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)]的活性。【结果】西花蓟马成虫体内的CarE,AchE,MFO,CAT和POD酶活性随着CO2浓度的升高而显著上升(P<0.05),其中800μL/L CO2浓度下CarE和MFO酶活性分别比400μL/L CO2浓度下增加了24.78%和16.05%;800μL/L CO2浓度下花蓟马成虫体内的CarE,MFO和CAT酶活性显著高于400μL/L CO2浓度下花蓟马成虫体内的相应酶活性,而AchE和POD酶活性在两种CO2浓度间差异不显著(P>0.05)。800μL/L CO2浓度下西花蓟马和花蓟马成虫体内的SOD酶活性均显著低于400μL/L CO2浓度下的相应蓟马酶活性(P<0.05),分别下降了65.22%和42.20%。【结论】CO2浓度升高是导致两种蓟马成虫体内CarE,MFO和SOD酶活性上升的主要原因,而AchE,CAT和POD酶活性的变化主要受蓟马种类的影响。两种蓟马可能通过改变体内解毒酶或保护酶的活性来适应高浓度CO2的环境。
【Objective】 The objective of this study is to elucidate the mechanism of Frankliniella occidentalis and F.intonsa, a native species of F. occidentalis, which is an invasive alien insect, and to investigate the mechanism of elevated CO2 in the atmosphere. 【Method】 Three kinds of detoxification enzymes [carboxylesterase (CarE), acetylcholinesterase (AChE), acetylcholinesterase (AchE) and microsomal multifunctional oxidase (MFO)] and 3 kinds of protective enzymes [superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)]. 【Result】 The results showed that the activities of CarE, AchE, MFO, CAT and POD were significantly increased with the increase of CO2 concentration (P <0.05). The CarE and MFO activities of 800 mg / L CO2 Which was 24.78% and 16.05% higher than that of 400μL / L CO2 concentration. The activities of CarE, MFO and CAT in 800f / L CO2 were significantly higher than those in adults of F. thrips at 400μL / L CO2 The activities of AchE and POD were not significantly different between the two CO2 concentrations (P> 0.05). The activities of SOD in F. thirties and F. thrips adults were significantly lower than those in 400 μL / L CO2 concentration (P <0.05) at 800 μL / L CO2 concentration, decreased by 65.22% and 42.20% respectively, . 【Conclusion】 The increase of CO2 concentration is the main reason for the increase of CarE, MFO and SOD activity in two adult T. thrips, while the change of AchE, CAT and POD activity is mainly affected by the species of Thrips. Both thrips may adapt to high CO2 concentrations by altering the activity of detoxification enzymes or protective enzymes in the body.