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将裙带菜配子体克隆包埋在含3%褐藻酸钙的胶球中,研究了预培养中的蔗糖浓度和培养时间,脱水过程中的脱水速率和胶球含水量以及化冻速度对配子体克隆的存活率和发育能力的影响,结果表明含裙带菜配子体克隆的胶球在0.3 mol/dm3的蔗糖培养基中培养9 h可以获得较高的存活率;胶球最适平均脱水速率为含水量的5%每小时,最适含水量为25%;较快的化冻速度可以显著提高配子体克隆的存活率,在优化条件下存活率可高达66%;经过冷冻保存的裙带菜配子体克隆在适合发育的条件下能够正常形成孢子体.
The gametophyte of Undaria pinnatifida was cloned into a gel ball containing 3% calcium alginate to study the sucrose concentration in pre-culture, the culture time, the dehydration rate in dehydration and the water content of glue ball, and the survival rate of the cloned gametophyte The results showed that the gelatin ball with the clone of the gametophyte of Undaria pinnatifida could survive for 9 h at 0.3 mol / dm3 in sucrose medium, and the optimum average rate of gelatinization was 5 % Per hour, the optimum moisture content is 25%; faster thawing speed can significantly improve the survival rate of gametocyte clones, under the optimized conditions, the survival rate can be as high as 66%; cryopreserved Undaria pinnatifida gametophyte clone suitable for development conditions Under the normal formation of sporophytes.