论文部分内容阅读
目的:研究清脑宣窍滴丸对急性脑缺血损伤的保护作用。方法:ig给予清脑宣窍滴丸(180,90,45 mg·kg-1剂量),每天1次,连续3 d,采用线栓法致大鼠大脑中动脉缺血再灌注(MCAO)模型,缺血2 h再灌注22 h,评分法评价神经损伤行为症状异常;放射免疫法检测血清中神经元特异性烯醇化酶(NSE)的活性;HE染色法观察脑组织病理形态的变化。结果:各给药组可不同程度改善损伤模型大鼠6 h和22 h的神经缺损症状,清脑宣窍滴丸180 mg.kg-1组与模型组比较有显著差异(P<0.05);清脑宣窍滴丸45,90,180 mg.kg-1组可降低模型大鼠NSE活性;病理结果显示,清脑宣巧滴丸组正常神经细胞数量明显增多,变性坏死组织范围缩小、程度减轻。结论:清脑宣窍滴丸对脑缺血有保护作用。
Objective: To study the protective effect of Qingnao Xuanqiao Drop Pills on acute cerebral ischemia injury. Methods: The rats were given chuannao Xuanqiao drop pills (180, 90, 45 mg · kg-1) once a day for 3 consecutive days. The middle cerebral artery occlusion (MCAO) , Ischemia-reperfusion at 2 h and reperfusion for 22 h. The scores of neurobehavioral symptoms were evaluated by radioimmunoassay. Serum neuron-specific enolase (NSE) activity was measured by radioimmunoassay. The pathological changes of brain tissue were observed by HE staining. Results: The neurological deficit symptoms of rats in the injury model at 6 h and 22 h were improved to some extent, and there was a significant difference (P <0.05) between Qinggan Xuanqiao Dropping Pill 180 mg · kg -1 group and the model group. Qingnaogi drop pills 45,90,180 mg.kg-1 group can reduce the NSE activity in rats; pathological results show that the number of normal brain cells Qingtong Xuanqiao dripping pills significantly increased, the scope of degenerative and necrotic tissue reduced, to a lesser extent. Conclusion: Qingnaoguanjiao drop pills have a protective effect on cerebral ischemia.