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选取平均体质量(150±2)g的埃及尼罗罗非鱼135尾,随机分为25℃、15℃和12℃共3个试验组,每组设3个重复组,每重复15尾,采用人工室内降温和电镜技术,探讨温度水平对埃及尼罗罗非鱼肝、脾和鳃细胞的影响。结果显示,埃及尼罗罗非鱼在15℃第16天时出现昏迷,在12℃第72小时时出现昏迷和死亡,埃及尼罗罗非鱼肝、脾和鳃细胞的损伤程度均随水温降低而增强,以12℃损伤最严重。与25℃相比,15℃时,埃及尼罗罗非鱼肝脏细胞边缘不清晰,形成小碎粒,脾脏组织边缘出现轻微溶解,鳃小片部分断裂,毛细血管轻微充血;12℃时,肝细胞线粒体数量减少,结构破碎化严重,内质网结构也呈现出破碎化的状态,脾脏组织边缘出现溶解,脾细胞出现与肝细胞类似的变化,鳃小片断裂严重,毛细血管充血严重。
A total of 135 Nile tilapia individuals with an average body mass of (150 ± 2) g were randomly divided into three groups (25 ℃, 15 ℃ and 12 ℃) Effects of temperature on the liver, spleen and gill cells of the Egyptian Nile tilapia were investigated by artificial indoor cooling and electron microscopy. The results showed that the Nile tilapia were comatose on the 16th day at 15 ℃. Coma and death occurred at the 72nd hour at 12 ℃. The damage of the liver, spleen and gill cells of the Egyptian Nile tilapia decreased with the decrease of water temperature Enhanced to 12 ℃ damage the most serious. Compared with 25 ℃, the edge of liver cells of Nile tilapia in Egypt was not clear at the edge of 25 ℃, forming small pieces of granule, the edge of spleen tissue appeared slight dissolution, the gill pieces were partially broken and the capillaries slightly hyperemia; at 12 ℃, Mitochondria decreased, the structure was severed severely, the endoplasmic reticulum structure also showed a state of fragmentation, spleen tissue edge dissolved, spleen cells appear similar changes with the liver cells, gill pieces severely broken, severe capillary congestion.