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目的研究浒苔多糖(Enteromorpha’s polysaccharide,EP)对2型糖尿病大鼠脂代谢影响。方法高糖高脂饲料喂养,结合注射小剂量链脲佐菌素(STZ)建立2型糖尿病大鼠模型,EP采用高(600mg/kg.bw)、中(300mg/kg.bw)、低(150mg/kg.bw)剂量进行干预。测定血脂水平、抗氧化指标;HE染色观察肝脏和胰腺组织结构;RT-PCR测定脂联素(adiponectin,APN)mRNA的表达水平。结果模型组精神萎靡,脱发、多饮、多食、多尿和体重减轻等T2DM症状明显,EP高、中、低剂量组2型糖尿病症状有所减轻。与空白组比较,模型组TC、TG、HDL-C和LDL-C明显较高(P<0.01)。与模型组比较,EP高、中、低剂量组TG明显较低(P<0.01),EP高、中剂量组TC、LDL-C明显较低(P<0.01),EP高、中剂量组HDL-C/TC比值较高(P<0.01)。EP高、中、低剂量组MDA明显较低(P<0.01),SOD较高(P<0.05),EP高、中剂量组GSH-Px较高(P<0.01,P<0.05),EP高、中剂量组脂肪APNmRNA表达较高(P<0.05),EP高、中、低剂量组肝脏细胞脂肪变性程度较轻,胰腺的病理损害较小。结论 EP对2型糖尿病大鼠的脂代谢具有一定的调节作用。[营养学报,2013,35(2):181-185]
Objective To investigate the effects of Enteromorpha polysaccharide (EP) on lipid metabolism in type 2 diabetic rats. Methods High glucose and high fat diet was used to establish type 2 diabetic rat model by injecting low dose of streptozotocin (STZ). The EP (600mg / kg.bw), middle (300mg / kg.bw) 150mg / kg.bw) dose intervention. The levels of lipids and antioxidants were measured. The structures of liver and pancreas were observed by HE staining. The mRNA expression of adiponectin (APN) was detected by RT-PCR. Results The symptoms of T2DM such as apnea, hair loss, polyphagia, polyphagia, polyuria and weight loss in model group were obvious. The symptoms of type 2 diabetes in EP high, middle and low dose groups were relieved. Compared with the blank group, TC, TG, HDL-C and LDL-C in model group were significantly higher (P <0.01). Compared with the model group, the levels of TC and LDL-C in the EP high, middle and low dose groups were significantly lower (P <0.01) and those in the high and middle dose EP groups were significantly lower (P <0.01) The C / TC ratio was higher (P <0.01). The levels of MDA in EP high, middle and low dose groups were significantly lower (P <0.01), higher in SOD (P <0.05), higher in EP and middle dose groups (P <0.01, P < (P <0.05). The fatty degeneration of liver cells in the high, middle and low dose groups of EP was lighter and the pathological damage of the pancreas was smaller. Conclusion EP can regulate lipid metabolism in type 2 diabetic rats. [Journal of Nutrition, 2013,35 (2): 181-185]