旨在探索一种分离细动脉平滑肌的方法，并研究其ATP敏感钾通道（KATP）的特性。采用链酶蛋白酶E消化及机械分离的方法分离细胞，以膜片钳技术之细胞贴附式和内面向外式记录KATP通过电流。该平滑肌的分离方法简单；细动脉平滑肌KATP通道在高钾平衡液中电导为（111．0±6.5）pS，门控动力学表现明显的ATP和电压依赖性，通道开放概率低，提示其对缺血、缺氧、酸中毒等病理条件下细动脉张力和组织血流分布的调节具有潜在的作用。 Aims To explore a method for the separation of arteriolar smooth muscle and study its ATP-sensitive potassium channel (KATP) properties. The cells were isolated by enzyme digestion and mechanical separation using streptavidin E, and the KATP currents were recorded by the patch-clamp technique and the patch-clamp technique. The smooth muscle separation method is simple. The arterial smooth muscle KATP channel is (111.0 ± 6.5) pS in high potassium balance solution. The kinetics of gating is obviously ATP and voltage-dependent, and the channel opening probability is low, Ischemia, hypoxia, acidosis and other pathological conditions under the regulation of arterial blood flow and the regulation of blood flow has a potential role.