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以转Bt基因抗虫棉选系为抗虫亲本 ,以各种类型的陆地棉常规品种 (系 )为非抗虫亲本杂交 ,研究了大量杂交组合不同世代群体在大田自然感虫条件下的抗棉铃虫性表现。结果表明 ,F1代均表现高抗棉铃虫 ,抗性不分离 ;同一杂交方式不同非抗虫亲本的F2 群体 ,以及年际间 ,抗株与不抗株的分离比例相差很大 ,说明亲本的遗传背景及不同的环境条件对转Bt基因抗虫棉杂交后代的抗性表现有较大影响 ;从F3～F5株行 ,随着世代的递增和连续进行抗性选择 ,抗性纯合株行的比例迅速上升 ,而分离株行不抗虫株的比例迅速降低 ,在田间棉铃虫自然协迫条件下 ,通过连续多代进行抗性选择 ,可使抗性迅速趋于稳定 ,至F5代可获得综合性状较为理想、抗性纯合、稳定的株系。结合前期研究结果及抗虫育种实践 ,提出了棉花转基因抗虫育种策略
The Bt transgenic cotton lines were used as insect-resistant parents, and the conventional varieties (lines) of all types of G. hirsutum were used as non-insect-resistant parents to study the resistance of different generations of large cross combinations to natural insects in field Cotton bollworm sexual performance. The results showed that the F1 generation showed high resistance to bollworm, the resistance was not separated; F2 population of different non-insect-resistant parents in the same hybridization pattern, and the separation ratio of resistant and non- Genetic background and different environmental conditions had a significant impact on the resistance performance of Bt transgenic cotton hybrid progeny. From F3 to F5 lines, with the increase of generations and continuous selection of resistance, The proportion of isolates did not decrease rapidly. Under the conditions of natural co-colonization by cotton bollworm in the field, resistance was rapidly stabilized through successive generations of selection, Obtained more comprehensive traits, resistance homozygous, stable strains. Combined with the previous research results and the practice of insect-resistant breeding, the strategy of transgenic cotton insect-resistant breeding was proposed