大花君子兰(Clivia miniata)是名贵的盆花,优良品种分株育苗,繁殖系数很低。1983年1月开始我们用组培育苗,选取蕾期花药、花丝、花柱、花柄、子房壁、胚,花葶、叶及根作试材。用Ms、N_6基本培养基,每升附加2,4-D0.5—2毫克,6-BA1毫克、NAA0-5毫克及蔗糖30—80克。接种后15—18天除花药、花瓣及花葶外,均产生了愈伤组织。取自繁殖器官的愈伤组织呈鲜红、红紫、黄绿或间色,取自营养器官的呈淡黄或淡绿色。培养基不适宜时 Clivia miniata (Clivia miniata) is a precious potted flowers, fine varieties of ramet nursery, breeding coefficient is very low. January 1983 began with our group of nursery seedlings, select the bud stage anthers, filaments, style, flower stalk, ovary wall, embryo, scabies, leaves and roots for the test material. With Ms, N_6 minimal medium, add 2,4-D0.5-2 mg, 6-BA1 mg, NAA0-5 mg and sucrose 30-80 g per liter. 15-18 days after inoculation in addition to anther, petals and scallops, have callus. Callus from reproductive organs was bright red, purple, yellow or green, taken from the vegetative organs pale yellow or light green. When the medium is not suitable