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目的 探讨过氧化物酶体增殖物激活受体 (PPAR- γ)的配体——噻格列酮对人胆囊上皮细胞炎症的调控。方法 分离培养胆囊上皮细胞 ,建立胆囊上皮细胞炎症模型 ,检测对照组和噻格列酮组培养液中 IL- 6、TNF-α值及细胞形态学变化。结果 成功分离培养胆囊上皮细胞并建立胆囊上皮细胞炎症模型 ,细胞最长可存活 2 5 d。炎症对照组细胞肿胀、胞膜不清 ,胞浆混浊 ,添加噻格列酮后 ,细胞炎性水肿有不同程度减轻 ,以 5 0 μmol/ m l组最明显。噻格列酮组 IL- 6值明显低于对照组 (P<0 .0 1) ,差别最大达 179.85 pg/ m l,抑制效应与浓度呈正相关关系。结论 噻格列酮能抑制胆囊上皮细胞炎症 ,有可能成为治疗急慢性胆囊炎的有效选择
Objective To investigate the regulation of peroxisome proliferator - activated receptor (PPAR - γ) ligand - thioglitazone on the inflammation of human gallbladder epithelial cells. Methods Gallbladder epithelial cells were isolated and cultured to establish the model of gallbladder epithelial cell inflammation. The levels of IL-6, TNF-α and the changes of cell morphology in the control group and the tridoglitazone group were detected. Results The gallbladder epithelial cells were successfully isolated and cultured to establish the gallbladder epithelial cell inflammation model. The longest cell could survive for 25 days. Inflammatory cells in the control group were swollen with unclear cell membrane and cytoplasm. After adding tiglitazone, inflammatory cell edema decreased to some extent, most notably in the group of 50 μmol / ml. The value of IL-6 in tiglitazone group was significantly lower than that in control group (P <0.01), and the difference was up to 179.85 pg / ml. The inhibitory effect was positively correlated with the concentration. Conclusion Troglitazone inhibits the inflammation of gallbladder epithelial cells and may be an effective treatment for acute and chronic cholecystitis