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目的:观察褪黑素对高糖高脂饲养大鼠胰岛素敏感性的影响。方法:将18只雄性SD大鼠(150~200g)随机分为正常组(CD组)与高糖高脂组(HFSD组),分别饲以正常饲料与高糖高脂饲料(53%基础饲料含蔗糖37%和猪油10%)喂养6个月后,给药治疗2个月;治疗期间,给褪黑素组(Mel组)每天腹腔注射药物褪黑素(Melatonin,Mel,4 mg/kg),对照组腹腔注射生理盐水。测定空腹血糖、胰岛素、甘油三酯(TG)、游离脂肪酸(FFA)、高密度脂蛋白胆固醇(HDL—C)水平,做口服葡萄糖耐量实验(OGTT)和胰岛素敏感性实验。结果:高糖高脂饮食可诱导SD大鼠产生胰岛素抵抗,引起SD大鼠空腹血糖、血清胰岛素、TG、FFA水平升高;胰岛素敏感性、HDL—C水平下降。褪黑素治疗后,胰岛素敏感性增强,改善了胰岛素抵抗。结论:Mel能降低高糖高脂饲养SD大鼠糖脂状况,提高胰岛素敏感性,改善胰岛素抵抗。
Objective: To observe the effect of melatonin on insulin sensitivity in high-sugar and high-fat diet rats. Methods: 18 male SD rats (150 ~ 200g) were randomly divided into normal group (CD group) and high glucose and high fat group (HFSD group), and were fed with normal diet and high glucose and high fat diet (53% basal diet Melatonin (Mel, 4 mg / kg) was given intraperitoneally to melatonin group (Mel group) for 6 months after feeding for 6 months. kg), the control group was injected with saline. Fasting blood glucose, insulin, triglyceride (TG), free fatty acid (FFA) and high density lipoprotein cholesterol (HDL-C) were measured to determine oral glucose tolerance (OGTT) and insulin sensitivity. Results: High-fat and high-fat diet induced insulin resistance in SD rats, which resulted in fasting blood glucose, serum insulin, TG and FFA levels in SD rats, insulin sensitivity and HDL-C levels decreased. After melatonin treatment, insulin sensitivity increased, improved insulin resistance. Conclusion: Mel can reduce the glucose and lipid status, improve insulin sensitivity and improve insulin resistance in SD rats fed with high glucose and high fat.