利用根癌农杆菌介导技术(ATMT)转化深绿木霉(Trichoderma atroviride)T23,共获得6个稳定的转化子。利用愈创木酚法对转化子进行筛选,发现3株转化子产漆酶能力显著高于出发菌株T23。在限碳培养基中,转化子TA5的漆酶酶活为25.3U/ml显著高于发菌株。研究发现TA5所产漆酶较适宜酸性条件,当pH为3.5时,漆酶活性最高。在温度为18℃时,漆酶活性最高。 Six stable transformants were obtained by transformation of Trichoderma atroviride T23 by Agrobacterium tumefaciens-mediated technique (ATMT). The transformants were screened by guaiacol method, and the ability of three transformants to produce laccase was significantly higher than that of the original strain T23. In carbon limited medium, the transformant TA5 laccase activity was 25.3U / ml significantly higher than the strain. The study found that laccase produced by TA5 is more suitable for acidic conditions, when pH is 3.5, laccase activity is the highest. At a temperature of 18 ° C, laccase activity is highest.