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以廊坊杨4号无菌苗为研究对象,确定了影响组培苗生长的关键因子及组培苗生长的最佳条件,并建立了稳定高效的再生体系。试验结果表明廊坊杨4号无菌苗的最适基本培养基是MS培养基,继代培养基为MS+6-BA 0.5 mg/L+IAA0.15 mg/L;不定芽分化培养基为MS+6-BA 0.5 mg/L+2,4-D0.05 mg/L;1/2MS+IBA 0.15 mg/L是其最适生根培养基组合。
Taking Langfang No.4 as a sterile seedling, the key factors affecting the growth of tissue culture seedlings and the optimum conditions for the growth of tissue culture seedlings were determined, and a stable and efficient regeneration system was established. The results showed that the optimum medium for the sterile seedlings of Yangfang No.4 was MS medium, the subculture medium was MS + 6-BA 0.5 mg / L + IAA 0.15 mg / L, and the adventitious bud differentiation medium was MS + 6-BA 0.5 mg / L + 2,4-D 0.05 mg / L; 1/2 MS + IBA 0.15 mg / L is the optimum rooting medium combination.