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目的:研究脊髓损伤(SCI)对大鼠骨组织中神经肽P物质表达的影响及中药方剂二仙汤对SCI后骨质疏松的治疗作用。方法:采用改良Allen’s法制作SCI大鼠模型,造模成功后将其随机分为模型组、二仙汤组及福善美组,同时设立HCI对照组和正常对照组。给予相应药物治疗8 w,记录不同时间点大鼠体质量及BBB评分变化,实验结束后取双侧股骨远端进行病理切片和免疫组化检测。结果:HCI对照组和正常对照组大鼠股骨结构完整,P物质阳性表达不明显;模型组出现骨小梁密度减低、小梁变细等骨质疏松的表现,P物质阳性表达明显增多;而二仙汤组和福善美组骨小梁数量及结构基本接近正常对照组,P物质阳性表达不明显。结论:与废用性骨质疏松不同,脊髓损伤后会引起损伤平面以下骨质疏松及神经肽P物质的增加,二仙汤对其有一定预防作用。
Objective: To study the effect of spinal cord injury (SCI) on the expression of neuropeptide P in rat bone and the therapeutic effect of Herbal Decoction for the treatment of osteoporosis after SCI. Methods: SCI rat model was made by modified Allen’s method. After successful modeling, the rats were randomly divided into model group, Erxian decoction group and Fushanmei group. HCI control group and normal control group were also established. The rats were given the corresponding drug for 8 weeks. The body weight and BBB scores of rats at different time points were recorded. The distal femur of bilateral femur was examined by pathology and immunohistochemistry after the experiment. Results: The structure of femur in HCI control group and normal control group were intact, while the positive expression of substance P was not obvious. In the model group, the density of trabecular bone and osteoporosis of trabeculae were decreased. The positive expression of substance P increased obviously. The quantity and structure of trabecular in Erxian decoction group and Fuxianmei group were close to those in normal control group, and the positive expression of substance P was not obvious. Conclusion: Different from disuse osteoporosis, spinal cord injury can cause osteoporosis and increase of neuropeptide substance P below the level of injury, and Erxiantang can prevent it.