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阿霉素作为一种广谱有效的抗肿瘤药物,由于具有严重的心脏毒性而限制了其临床使用。心肌线粒体钙运输系统包括钙摄取和钙释放两部分,线粒体钙运输系统的功能之一是对氧化磷酸化过程有重要调节作用。有关阿霉素对心肌线粒体钙运输的影响目前国外仅限于心肌线粒体钙摄取方面的体外试验资料。有关阿霉素对心肌线粒体钙摄取、钙释放、线粒体游离钙、线粒体总钙的体内作用观察,国内外尚鲜见文献报道。本工作用原子吸收分光光度法和Fura 2AM荧光指示剂分别测定了不同药物处理下大鼠心肌线粒体钙摄取、钙释放、游离钙、总钙。观察了阿霉素对心肌线粒体钙运输的影响以及与心肌线粒体游离钙和心肌线粒体总钙
Adriamycin, as a broad-spectrum anti-tumor drug, has its clinical implications limited due to its severe cardiotoxicity. Myocardial mitochondrial calcium transport system includes two parts, calcium intake and calcium release. One of the functions of mitochondrial calcium transport system is to regulate oxidative phosphorylation. The impact of doxorubicin on myocardial mitochondrial calcium transport At present, foreign countries are limited to myocardial mitochondrial calcium uptake in vitro test data. Adriamycin on myocardial mitochondrial calcium uptake, calcium release, mitochondrial free calcium, total mitochondrial calcium in vivo effects observed at home and abroad is still rarely reported in the literature. In this work, the mitochondrial calcium uptake, calcium release, free calcium and total calcium in myocardium were measured by atomic absorption spectrometry and Fura 2AM fluorescent indicator respectively. The effects of doxorubicin on myocardial mitochondrial calcium transport as well as the relationship between myocardial mitochondrial free calcium and myocardial mitochondrial total calcium