目的建立高效液相色谱法测定还阳参药材中绿原酸的方法。方法采用Sino Chrom DDS-BP色谱柱(250mm×4.6mm,5μm),流动相为乙腈-0.4%磷酸水溶液(13:87),体积流量1.0mL/min,检测波长327nm,柱温25℃。结果绿原酸进样量在0.046～0.644μg与峰面积呈良好的线性关系(r=1),样品平均加样回收率为101.9%,RSD值为1.22%(n=6);检出限和定量限分别为0.01ng和0.04ng。结论此方法简便、准确、重复性好,可用于还阳参药材的质量控制。 Objective To establish a HPLC method for the determination of chlorogenic acid in Radix Salviae Miltiorrhizae. Methods The chromatographic column of Sino Chrom DDS-BP (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.4% phosphoric acid solution (13:87), the volume flow rate was 1.0 mL / min, the detection wavelength was 327 nm and the column temperature was 25 ℃. Results The linear range (0.046-0.684 μg) of chlorogenic acid was linear with the peak area (r = 1). The average recovery was 101.9% and the RSD was 1.22% (n = 6). The detection limit And the limit of quantification were 0.01 ng and 0.04 ng, respectively. Conclusion This method is simple, accurate, reproducible, and can be used for the quality control of Radix Salviae Miltiorrhizae.