论文部分内容阅读
本研究以过表达截形苜蓿类型ⅠH~+-焦磷酸酶编码基因MtVP1的转基因马铃薯为材料,对其抗旱性进行了检测。结果显示:(1)在正常生长条件下,与野生型植株相比,两个转基因株系的叶片相对含水量、叶绿素含量、丙二醛含量和超氧化物歧化酶活性差异均不显著,而脯氨酸含量均高于野生型植株,其中OE2株系脯氨酸含量显著高于野生型植株。随着干旱胁迫的持续,两个转基因株系的叶片相对含水量、叶绿素含量的下降趋势平缓,其含量均显著高于野生型对照,且两个转基因株系的脯氨酸含量和SOD活性显著高于野生型对照,而转基因株系的MDA含量显著低于野生型植株。(2)转MtVP1马铃薯表现出表皮毛增多的新表型,茎和叶表面的表皮毛明显比野生型植株多。以上结果表明,Mt VP1基因的过表达提高了马铃薯的渗透调节能力,从而增强了马铃薯的抗旱性;还表明植物类型ⅠH~+-焦磷酸酶基因可能参与调控其他的代谢通路,具有更复杂的生理功能。
In this study, the transgenic potatoes overexpressing MtPH of truncated alfalfa type ⅠH ~ + - pyrophosphatase were tested for their drought resistance. The results showed that: (1) Under normal growth conditions, the relative water content, chlorophyll content, malondialdehyde content and superoxide dismutase activity of two transgenic lines were not significantly different from the wild type plants Proline content were higher than the wild-type plants, including OE2 strain proline content was significantly higher than the wild-type plants. With the continuous drought stress, the leaf relative water content and chlorophyll content of the two transgenic lines tended to decline slowly, and their content was significantly higher than that of the wild-type control, and the proline content and SOD activity of the two transgenic lines were significant Higher than the wild-type control, while the transgenic lines MDA content was significantly lower than the wild-type plants. (2) Transgenic MtVP1 showed a new phenotype with more epidermal hairs, with more epidermal hairs on stem and leaf surfaces than wild-type plants. The above results indicated that overexpression of Mt VP1 increased the ability of osmotic adjustment of potato and thus enhanced the drought resistance of potato. It also indicated that the plant type ⅠH ~ + - pyrophosphatase gene might be involved in the regulation of other metabolic pathways, with more complex Physiological function.