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目的探讨头颈部鳞状细胞癌(HNSCC)中细胞周期素依赖蛋白激酶5(CDK5)及上皮间充质转化(EMT)相关蛋白N-钙黏蛋白(N-cadherin)、波形蛋白(vimentin)及E-钙黏蛋白(E-cadherin)表达,以及CDK5的表达与患者预后的关系。方法免疫组化方法检测55例HNSCC患者癌组织中CDK5及EMT相关蛋白的表达情况;分析不同临床病理特征患者CDK5及EMT相关蛋白表达的差异,CDK5与EMT相关蛋白表达的相关性,以及CDK5表达与患者生存之间的关系。结果 CDK5高表达率在有淋巴结转移患者中高于无淋巴结转移患者(91.67%vs30.23%,P<0.05),在T3~T4期患者中高于T1~T2期患者(85%vs20%,P<0.05);有淋巴结转移患者N-cadherin、Vimentin高表达率高于无淋巴结转移的患者(75.00%vs6.98%;91.67%vs27.91%,均P<0.05),E-cadherin高表达率低于无淋巴结转移的患者(8.33%vs86.05%,P<0.05);CDK5与N-cadherin、Vimentin表达呈正相关,与E-cadherin表达呈负相关(r_s分别为0.512、0.443、-0.363,均P<0.01);CDK5高表达患者3年生存率低于低表达患者(37.5%vs87.0%,Logrank,χ~2=12.678,P<0.01)。结论 CDK5及EMT相关蛋白在转移性HNSCC中异常表达;CDK5的表达状态对预测HNSCC患者的预后有一定价值。
Objective To investigate the expression of CDK5 and N-cadherin, vimentin in epithelial cell carcinoma of head and neck squamous cell carcinoma (HNSCC) And E-cadherin (E-cadherin) expression, and CDK5 expression and prognosis of patients. Methods Immunohistochemical method was used to detect the expression of CDK5 and EMT-related proteins in 55 cases of HNSCC. The differences of CDK5 and EMT-related proteins, CDK5 and EMT-related proteins in different clinical and pathological features were analyzed, and the expression of CDK5 Relationship with patient’s survival. Results The high expression rate of CDK5 in patients with lymph node metastasis was higher than that in patients without lymph node metastasis (91.67% vs 30.23%, P <0.05), higher in patients with T3 ~ T4 than in patients with T1 ~ T2 (85% vs20%, P < 0.05). The high expression rates of N-cadherin and Vimentin in patients with lymph node metastasis were higher than those without lymph node metastasis (75.00% vs6.98%; 91.67% vs27.91%, all P <0.05), and the high expression rate of E-cadherin There was a positive correlation between the expression of CDK5 and N-cadherin and Vimentin, but negatively correlated with the expression of E-cadherin (r_s = 0.512, 0.443, -0.363, respectively) in patients without lymph node metastasis (8.33% vs 86.05%, P < P <0.01). The 3-year survival rate of patients with high expression of CDK5 was lower than that of patients with low expression (37.5% vs 87.0%, Logrank, χ ~ 2 = 12.678, P <0.01). Conclusion CDK5 and EMT-related proteins are abnormally expressed in metastatic HNSCC. The expression of CDK5 is of value in predicting the prognosis of patients with HNSCC.