论文部分内容阅读
[Objective]The research aimed to establish the detection method of isoorientin from different parts of Gentianna straminea Maxim.by reversed phase-HPLC.[Method]The chromatographic conditions were as follows:Hypersil ODS column (4.6 mm×250 mm,5μm),40% methanol-H2O (containing 0.02% phosphoric acid) as a mobile phase,with flow rate of 1 ml/min,column temperature at 35℃,detection wavelength at 254 nm.[Result]Under the chromatographic conditions,isoorientin and other components reached the baseline separation.The linear range was 0.089 9-0.539 μg,r=0.999 9.The addition recovery was 99.7%,with RSD of 0.49%.[Conclusion]The method is rapid and accurate and the results are reliable.
[Objective] The research aimed to establish the detection method of isoorientin from different parts of Gentianna straminea Maxim.by reversed phase HPLC. [Method] The chromatographic conditions were as follows: Hypersil ODS column (4.6 mm × 250 mm, 5 μm) 40% methanol-H2O (containing 0.02% phosphoric acid) as a mobile phase with flow rate of 1 ml / min, column temperature at 35 ° C, detection wavelength at 254 nm. [Result] Under the chromatographic conditions, isoorientin and other components reached the baseline separation. The linear range was 0.089 9-0.539 μg, r = 0.999 9. The addition recovery was 99.7% with RSD of 0.49%. [Conclusion] The method is rapid and accurate and the results are reliable.