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研究结果表明,在分蘖、孕穗和抽穗期,供试杂交稻组合的功能叶片中的氮浓度、可溶性蛋白质和叶绿素含量明显高于常规稻.在其氮素代谢过程中,NR,GS,Fd-GOGAT,GPT和GOT酶活力,在供试品种间的差异是:汕优64>汕优6号>浙丽1号>秀水48,氮素营养条件的变化对此差异无影响,在杂变稻与常规稻之间,Fd-GOGAT酶活力和NR酶活力差异最大(分别达到50~70%和40~70%),而GPT酶活的差异较小.结果还表明,在不同水稻品种的功能叶中,上述5种酶的活力,与叶中可溶性蛋白质含量及全氮量均呈积显著正相关,这说明在杂交稻叶片中,氮素同化和蛋白质合成的酶系统,其活力优于常规稻的该酶系统,它们是杂交稻吸收利用氮素能力强的重要生理基础之一.
The results showed that the nitrogen concentration, soluble protein and chlorophyll content in functional leaves of tested hybrid rice combinations were significantly higher than those of conventional rice at tillering, booting and heading stages.N, GS, Fd- GOGAT, GPT and GOT activities. The differences among tested cultivars were as follows: Shanyou 64> Shanyou 6> Zheli 1> Xiushui 48. The changes of nitrogen nutrition had no effect on this difference, Compared with the conventional rice, Fd-GOGAT enzyme activity and NR enzyme activity had the highest difference (50-70% and 40-70%, respectively), while the difference of GPT enzyme activity was less.The results also showed that in the function of different rice varieties There was a significant positive correlation between the activities of these five enzymes and soluble protein content and total nitrogen content in the leaves, indicating that the activity of the enzyme system of nitrogen assimilation and protein synthesis in hybrid rice leaves is superior to conventional The enzyme system of rice, which is one of the important physiological basis for hybrid rice to absorb and utilize nitrogen.