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本文重点研究用中药脱除大肠杆菌携带的Ap~T、TC~T耐药性质粒。我们用分子生物学方法对几种中药脱质粒进行了试验,证实娄仁对耐药性质粒有肯定的治愈(消除)作用,虽然找到中药不多,但开辟了中药与分子生物学结合为临床服务的途径。 本文根据多年来医院中多重耐药菌株引起的感染性疾病大大增多,除革兰氏阳性葡萄球菌外,还有肠道杆菌及其它革兰氏阴性菌多数由质粒介导耐药性,这种现象导致增加了治疗上的困难,为此,我们曾多方寻求脱除耐药质粒的办法,以增强细菌对药物的敏感性,提高治愈率,现将结果简报如下。 结果 一、将每管24小时培养物分别按5μl涂布在普通LB及含AP、TC平板上后,观察到实验组管菌在普通LB及含AP、TC平板上,其普通LB平板上苗落数明显多于含AP、TC平板上生长的菌落数(P<0.05)。其差异在统计学上有显著意义。面对照组1～5管的菌,生长在普通LB平板上的菌落数与含AP、TC平板上
This article focuses on the use of traditional Chinese medicine to remove E. coli carrying Ap ~ T, TC ~ T drug resistance plasmid. We used molecular biological methods to test the degranulation of several traditional Chinese medicines, and confirmed that Coix seed has a positive healing (eliminating) effect on drug-resistant plasmids. Although we found that there are not many traditional Chinese medicines, we have developed a combination of traditional Chinese medicine and molecular biology as clinical The way to service. This article has greatly increased the number of infectious diseases caused by multidrug-resistant strains in hospitals over the years. In addition to gram-positive staphylococci, there are mostly plasmid-mediated drug resistance of enterobacteriaceae and other gram-negative bacteria. The phenomenon has led to increased treatment difficulties. For this reason, we have sought to eliminate the resistance plasmids in order to increase the sensitivity of bacteria to drugs and increase the cure rate. The results are now reported below. As a result, after 24 hours culture of each tube was coated on common LB and AP- and TC plates with 5 μl, the experimental group was observed to grow on ordinary LB and AP- and TC-containing plates. The number of colonies was significantly higher than the number of colonies grown on plates containing AP and TC (P<0.05). The difference was statistically significant. The control group 1 to 5 tubes of bacteria, the number of colonies grown on ordinary LB plate and AP, TC plate