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应用RAPD分子标记技术对圆斑星鲽(Verasper variegatus)和条斑星鲽(Verasper moseri)的养殖群体进行群体遗传多样性分析。每个群体各取鱼30尾,并从78条随机引物中筛选出20条用于群体遗传学研究。两个群体共扩增出218条DNA片段,大多数片段大小为250~1500 bp,其中圆斑星鲽185条,条斑星鲽183条。多态片段比例分别为56.76%和64.48%,Nei的多样性指数和Shannon的信息指数分别为0.2322和0.2781、0.3391和0.3989。这两种星鲽养殖群体间的遗传相似性系数和遗传距离分别为0.6720和0.3975。
The population genetic diversity of Verasper variegatus and Verasper moseri was analyzed by RAPD molecular marker technique. Thirty fish were taken from each group, and 20 of 78 random primers were selected for population genetics research. A total of 218 DNA fragments were amplified in the two populations, most of which ranged from 250 to 1500 bp in size, of which 185 were serrated and 183 were striped. The proportions of polymorphic fragments were 56.76% and 64.48% respectively. The Nei diversity index and Shannon’s information index were 0.2322 and 0.2781, 0.3991 and 0.3989, respectively. The genetic similarity coefficient and genetic distance between the two starburst farmed populations were 0.6720 and 0.3975, respectively.