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对鸡传染性喉气管炎病毒（ＩＬＴＶ）的鸡胚、鸡胚肾细胞等培养系统进行研究；对病毒包涵体、病毒形态进行观察；对病毒毒价进行测定。结果，病毒在鸡胚绒毛尿囊膜产生痘斑和坏死灶，鸡胚发育不良并致死；鸡胚肾细胞稳定地产生合胞体等细胞病变；绒毛尿囊膜切片经Ｈ－Ｅ梁色，可观察到紫红色嗜酸性核内包涵体充满细胞核；病毒细胞培养物粗提后用负染法电镜观察，绒毛尿囊膜用超薄切片法电镜观察，均可见到病毒颗粒，病毒直径约２４０～３００ｎｍ，核衣壳直径约１００～１４０ｎｍ，外有圆形或椭圆形囊膜；经微量培养系统测定，病毒毒价为１０６．１２ＴＣＩＤ５０／０．０５ｍＬ。
The culture system of chick embryo and chick embryo kidney cells of infectious laryngotracheitis virus (ILTV) was studied. The virus inclusion body and virus morphology were observed. The virus titer was determined. As a result, the virus produced chickenpox chorioallantoic membrane lesions and necrosis, chick embryo dysplasia and lethal; chicken embryo kidney cells stably syncytiotrophoblastic and other cytopathic lesions; chorioallantoic membrane section by H-E beam color can be The purple eosinophilic nuclear inclusions were observed to be filled with nuclei. The virus cell culture was observed with negative staining electron microscopy and villous allantoic membrane by ultra-thin section electron microscopy. All the virus particles were observed. The virus diameter was about 240 ~ The diameter of the nucleocapsid was about 100-140 nm, with a round or oval capsule outside. The drug price of the virus was 106.12 TCID50 / 0.05 mL as determined by the micro culture system.