目的　探索载破伤风类毒素 (TT)蛋白的聚合物微球作为疫苗在动物体内的免疫效果。方法　采用溶剂蒸发技术制备包裹TT的聚乳酸及聚乳酸 /聚乙醇酸共聚微球 (PLGTTMS) ,并以ELISA法和小鼠中和法测定免疫小鼠和豚鼠的血清中和抗体。结果　用这些载TT聚合物微球免疫豚鼠和小鼠后 ,所诱生的抗体反应大大高于未加佐剂的TT疫苗 ,且由大粒径和小粒径微球混和组成的PLATTMS所诱生的抗体反应呈现出明显的加强效应。用 2 0LD50 的破伤风毒素攻击后 ,氢氧化吸附的TT和包裹TT的不同聚合物微球免疫的小鼠均获得 10 0 %的保护率。结论　以聚乳酸及其与聚乙醇酸共聚微球为载体的破伤风类毒素蛋白疫苗在动物体内诱生的免疫反应显著高于无佐剂疫苗 ,这为疫苗的开发提供了一条新途径。 OBJECTIVE: To investigate the immunogenicity of tetanus toxoid (TT) -containing polymer microspheres as a vaccine in animals. Methods Poly (DL-lactic acid) and poly (DL-lactide-co-glycolic acid) (PLGTTMS) encapsulated TT were prepared by solvent evaporation technique. Serum neutralizing antibodies of immunized mice and guinea pigs were determined by ELISA and mouse neutralization method. Results Immunization of guinea pigs and mice with these TT-loaded polymeric microspheres resulted in significantly higher antibody responses than the unadjuvanted TT vaccine and was induced by PLATTMS, a blend of large and small particle size microspheres The antibody response showed a significant strengthening effect. After challenge with 20 LD50 of tetanus toxin, mice immunized with both hydroxide-adsorbed TT and TT-coated different polymer microspheres achieved a 100% protection. CONCLUSION: The immune response induced by polylactic acid and its conjugate polyglycolic acid microspheres with tetanus toxoid vaccine was significantly higher in animals than in non-adjuvanted vaccine, which provided a new way for vaccine development.