AIM:Human zinc finger protein 191 (ZNF191) was clonedand characterized as a Krüppel-like transcription factor,whichmight be relevant to many diseases such as liver cancer,neuropsychiatric and cardiovascular diseases.Althoughprogress has been made recently,the biological function ofZNF191 remains largely unidentified.The aim of this studywas to establish a ZNF191 transgenic mouse model,whichwould promote the functional study of ZNF191.METHODS:Transgene fragments were microinjected intofertilized eggs of mice.The manipulated embryos weretransferred into the oviducts of pseudo-pregnant femalemice.The offsprings were identified by PCR and Southernblot analysis.ZNF 191 gene expression was analyzed byRT-PCR.Transgenic founder mice were used to establishtransgenic mouse lineages.The first generation (F1) andthe second generation (F2) mice were identified by PCRanalysis.Ten-week transgenic mice were used forpathological examination.RESULTS:Four mice were identified as carrying copies ofZNF191 gene.The results of RT-PCR showed that ZNF191gene was expressed in the liver,testis and brain in one ofthe transgenic mouse lineages.Genetic analysis of transgenicmice demonstrated that ZNF191 gene was integrated intothe chromosome at a single site and could be transmittedstably.Pathological analysis showed that the expression ofZNF 191 did not cause obvious pathological changes inmultiple tissues of transgenic mice.CONCLUSION:ZNF 191 transgenic mouse model wouldfacilitate the investigation of biological functions of ZNF191in vivo. AIM: Human zinc finger protein 191 (ZNF191) was clonedand characterized as a Krüppel-like transcription factor, whichmight be relevant to many diseases such as liver cancer, neuropsychiatric and cardiovascular diseases. Though there has been made recently, the biological function of ZF191 remains largely unidentified The aim of this study was to establish a ZNF191 transgenic mouse model, which could promote the functional study of ZNF191.METHODS: Transgene fragments were microinjected intofertilized eggs of mice. Manipulated embryos weretransferred into the oviducts of pseudo-pregnant femalemice.The offsprings identified by PCR and Southern blot analysis. ZNF 191 gene expression was analyzed by RT-PCR. Transgenic founder mice were used to establish transgenic mouse lines. The first generation (F1) and the second generation (F2) mice were identified by PCR analysis. Ten-week transgenic mice were used forpathological examination.RESULTS: Four mice were identified as carrying copies ofZNF191 gene.The results of RT-PCR showed that ZNF191gene was expressed in the liver, testis and brain in one of the transgenic mouse lines. Genetic Analysis of transgenic mouseice that that ZNF191 gene was integrated intothe chromosome at a single site and could be transmittedstably. Pathological analysis showed that the expression ofZNF 191 did not cause significant pathological changes inmultiple tissues of transgenic mice. CONCLUSION: ZNF 191 transgenic mouse model would allow a investigation of biological functions of ZNF191 in vivo.