论文部分内容阅读
Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg~(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg~(2+) in real water samples and mapping of intracellular Hg~(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.
Functional nucleic acids (FNAs) -based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled (fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids (AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease (DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg ~ (2+) are achieved. This robust assay allows for sensitive and selective detection of Hg ~ (2+ ) in real water samples and mapping of intracellular Hg ~ (2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.