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目前,国内大多数开展人类细胞培养和染色体标本制作的实验室已有了一套比较固定的方法,但是少数刚开始工作的实验室常常会碰到这样或那样的问题。本文就有关问题作些分析,并提出一些解决问题的措施。 1.染色体分裂相少这种情况是大多数刚开展这项工作的实验室最常见的问题,是由于培养基的质量所引起的。在人血细胞培养中所用的培养基通常由三种主要成分组成:一种是血清;一种是综合人工营养液;另一种是PHA(植物凝血素)。PHA可在配制培养基时加入其内,也可临时按比例
At present, most domestic laboratories that carry out human cell culture and chromosome sample preparation have a relatively fixed method, but a few laboratories that have just started work often encounter such problems. This article makes some analysis of relevant issues and proposes some solutions to the problems. 1. Chromosomal fragmentation is rare This situation is the most common problem in most laboratories who have just started this work, because of the quality of the medium. The culture medium used in human blood cell culture is usually composed of three main components: one is serum; the other is a comprehensive artificial nutrient solution; the other is PHA (phytohaemagglutinin). PHA can be added to the medium when it is formulated, or it can be temporarily proportional