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目的调查云南省兰坪县人、鼠间肾综合征出血热(HFRS)的感染情况,为HFRS预防控制提供指导。方法鼠类肺脏组织中汉坦病毒抗原检测采用直接免疫荧光法,人和鼠血清中汉坦病毒抗体检测采用间接免疫荧光法。结果兰坪县3个乡镇共捕获5属158只啮齿动物,其中居民区中褐家鼠的构成比为91.09%,为优势种;野外高山姬鼠的构成比为63.16%,为优势种。3个乡镇中仅见中排乡捕获的褐家鼠(Rattusnorvegicus)汉坦病毒抗原的感染率为7.69%(5/65),臭鼠句鼠青(Suncusmurinus)为25.00%(1/4),其他捕获的动物中未查出汉坦病毒抗原;宿主动物血清中汉坦病毒IgG抗体阳性率为12.20%(5/41),其中褐家鼠的阳性率为12.50%(5/40);人群中汉坦病毒IgG抗体阳性率为7.00%(3/43)。结论从兰坪县人群血清中查到汉坦病毒抗体,在宿主动物肺脏组织检测出汉坦病毒抗原,血清中查出汉坦病毒抗体,首次证实云南省兰坪县存在肾综合征出血热自然疫源地,为肾综合征出血热在该地区的预防控制提供了科学依据。
Objective To investigate the prevalence of HFRS in Lanping County of Yunnan Province and provide guidance for the prevention and control of HFRS. Methods Direct immunofluorescence assay was used to detect Hantavirus antigen in murine lung tissue, and indirect immunofluorescence assay was used to detect Hantaviruses in human and mouse serum. Results A total of 158 genera and 5 rodents were captured in 3 townships in Lanping County. Among them, the ratio of Rattus norvegicus in residential areas was 91.09%, which was the dominant species. The composition ratio of wild alpine Apodemus was 63.16%, which was the dominant species. The infection rates of Rattus norvegicus hantavirus antigen were 7.69% (5/65), 25.00% (1/4), and other Hantavirus antigen was not detected in the captured animals; the positive rate of Hantavirus IgG in the serum of the host animals was 12.20% (5/41), of which Rattus norvegicus was 12.50% (5/40); in the crowd The positive rate of Hantavirus IgG was 7.00% (3/43). Conclusion Hantavirus antibody was detected in the serum of Lanping County, Hantavirus antigen was detected in the lungs of host animals, and Hantavirus was detected in serum. It was confirmed for the first time that there was natural hemorrhagic fever with renal syndrome in Lanping County of Yunnan Province Focal areas for the prevention and treatment of hemorrhagic fever with renal syndrome provide a scientific basis for the prevention and control in the area.