AIM:To evaluate the effects of honey on bacterial translocation and intestinal villus histopathology in experimental obstructive jaundice. METHODS:Thirty Wistar-Albino rats were randomly divided into three groups each including 10 animals:group Ⅰ, sham-operated;group Ⅱ,ligation and section of the common bile duct (BDL);group Ⅲ,bile duct ligation followed by oral supplementation of honey (BDL+honey) 10 g/kg per day.Liver, blood,spleen,mesenteric lymph nodes,and ileal samples were taken for microbiological,light and transmission electrone microscopic examination. RESULTS:Although the number of villi per centimeter and the height of the mucosa were higher in sham group,there was no statistically significant difference between sham and BDL+honey groups (P>0.05).On the other hand, there was a statistically significant difference between BDL group and other groups (P0.05).BDL group had significantly higher rates of bacterial translocation as compared with sham and honey groups. Bacterial translocation was predominantly detected in mesenteric lymph nodes. CONCLUSION: Supplementation of honey in presence of obstructive jaundice ameliorates bacterial translocation and improves ileal morphology.