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目的 确认未成熟脑对惊厥性脑损伤可能具有的特别承受能力。方法 用美解眠诱发健康幼龄和成年期Wistar大鼠持续惊厥发作。通过比较惊厥持续时间、程度及惊厥后死亡情况 ,光镜下进行海马、颞叶、顶叶皮层坏死神经元计数 ,判断不同龄大脑对惊厥的敏感性及耐受性 ;并使用电镜确认神经元超微结构病变。结果 (1)成年鼠仅有 <2 0min的惊厥发作 ,而幼鼠惊厥持续状态发生率为 71.4 %。 (2 )惊厥发作后幼鼠死亡发生在 (44± 2 4 )min ,而成年鼠死于 (7± 5 )min。 (3)成年及幼年鼠惊厥后神经元损伤均主要发生在海马区。成年鼠惊厥持续时间皆≤ 2 0min ,该区的坏死神经元高达 (2 2 7± 34) / 80 0个 ;幼龄鼠惊厥持续≤ 2 9min者 ,坏死神经元仅 (6 0± 2 1) / 80 0个 ,惊厥持续 30～ 5 9min者 ,为 (16 7± 5 1) / 80 0个。结论 (1)不同成熟期大脑对致惊剂的敏感性差异有显著性。年龄越幼 ,敏感性越高 ,越容易发生长时程惊厥或惊厥持续状态 ;(2 )发育期脑对长时程惊厥发作的承受力强 ;(3)惊厥发作引起颞叶、海马及齿状回等脑区选择性神经元变性和死亡。病变程度除与年龄明显相关外 ,还取决于惊厥发作的持续时间
Objective To confirm the special tolerance that immature brains may have in patients with convulsive brain injury. Methods US sculpting was induced in healthy young and adult Wistar rats with seizures. By comparing the duration of convulsion, the degree of death and the post-convulsion seizure, the neurons in hippocampus, temporal lobe and parietal cortex were counted under light microscope to determine the sensitivity and tolerability of convulsions in different age brains. Electron microscopy was used to confirm the neuronal Ultrastructural lesions. Results (1) The convulsive seizures were only <20 min in adult rats, and 71.4% in young rats. (2) Death occurred in young rats (44 ± 2 4) min after convulsion seizures and in adult rats (7 ± 5) min. (3) Adult and juvenile rats with neuronal damage after convulsion mainly occurred in the hippocampus. The duration of convulsions in adult rats was ≤20 min, the number of necrotic neurons in this area was as high as (22.7 ± 34) / 80 0, the duration of convulsions lasting ≤ 29 min in young rats was (60 ± 21) / 80 0, seizures continued for 30 ~ 59 min were (16 7 ± 5 1) / 80 0. Conclusions (1) There are significant differences in sensitivities to stunned agents in different mature brain. Older age, the higher the sensitivity, the more prone to long-term seizures or convulsions continued state; (2) Developmental brain tolerance to long-term seizures; (3) seizures caused by temporal lobe, hippocampus and teeth Retrospective brain regions such as selective neuronal degeneration and death. In addition to the degree of disease significantly correlated with age, but also depends on the duration of seizures