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目的探讨DNA修复基因X线修复交叉互补基因1(XRCC1)399与人类着色性干皮病基因D(XPD)751基因多态性与苯乙烯致周围血淋巴细胞损伤的关系。方法 选取山东省某机车外壳生产厂126名苯乙烯作业人员为接触组,150名非苯乙烯作业人员为对照组。应用限制性片段长度多态性聚合酶链反应技术检测患者XRCC1 399位点和XPD 751位点的基因多态性,分析其与苯乙烯致周围血淋巴细胞损伤的关系。结果 XRCC1 399 Arg/Gln分布主要以Gln/Gln和Arg/Gln基因型为主,Arg/Arg基因型仅出现在少数人群中。XPD 751 Lys/Gln主要以Lys/Lys基因型为主,未发现Gln/Gln基因型者,2种基因的基因型均符合Hardy-Weinberg平衡。接触组携带XRCC1 399 Arg/Gln和Gln/Gln基因型的个体周围血淋巴细胞双核微核率低于Arg/Arg基因型者,差异有统计学意义(P<0.05),携带XPD 751 Lys/Gln基因型的个体周围血淋巴细胞双核微核率高于Lys/Lys基因型者,差异有统计学意义(P<0.05)。结论 XRCC1 399和XPD751基因多态性可能与苯乙烯致周围血淋巴细胞损伤有关系。
Objective To investigate the relationship between DNA repair gene XRCC1 399 and human CDC gene D (XPD) 751 gene polymorphism and styrene induced peripheral blood lymphocyte injury. Methods Twenty-six styrene workers in a locomotive shell factory in Shandong Province were selected as the contact group and 150 non-styrene workers as the control group. The polymorphism of XRCC1 399 and XPD 751 loci were detected by restriction fragment length polymorphism (PCR) and the relationship between them and styrene-induced peripheral blood lymphocyte injury was analyzed. Results The distribution of XRCC1 399 Arg / Gln was mainly found in the genotypes of Gln / Gln and Arg / Gln. The Arg / Arg genotype only appeared in a few people. The main Lys / Lys genotypes of XPD 751 Lys / Gln were genotypes of Gln / Gln. The genotypes of the two genes were in accordance with Hardy-Weinberg equilibrium. There were significant differences (P <0.05) between the two groups in the contact group and those carrying the XRCC1 399 Arg / Gln and Gln / Gln genotypes and the peripheral blood lymphocytes with XPD 751 Lys / Gln The genotypes of peripheral blood lymphocyte dual-core micronucleus rate was higher than Lys / Lys genotype, the difference was statistically significant (P <0.05). Conclusion The polymorphisms of XRCC1 399 and XPD751 may be related to the damage of peripheral blood lymphocytes induced by styrene.