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以五年生人参根组织为试验材料,提取总RNA,反转录合成cDNA,利用RT-PCR法对人参鲨烯环氧酶(SQE)基因的cDNA进行克隆及序列分析,初步探讨人参皂苷生物合成途径中的影响因子。结果表明:获得人参SQE基因全长片段大小为1 636 bp,开放阅读框长1 611 bp,编码536个氨基酸残基,与其他植物核苷酸序列具有较高同源性,其中与三七、龙牙惚木、刺五加同源性分别为98%、96%、90%。
Five-year-old ginseng root tissue was used as experimental material to extract total RNA and reverse transcribed into cDNA. The cDNA of ginseng squalene epoxidase (SQE) gene was cloned and sequenced by RT-PCR. The ginsenoside biosynthesis Impact factors in the pathway. The results showed that the full-length fragment of SQE gene was 1 636 bp in length and 1 611 bp in open reading frame encoding 536 amino acid residues, which shared high homology with other plant nucleotide sequences. Among them, Mori, Acanthopanax homology was 98%, 96%, 90%.