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目的分析江苏地区汉族人群白细胞分化抗原14(CD14)基因启动子C(-260)T多态性与冠心病之间的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析193例经冠脉造影确诊的冠心病患者及225例健康对照者CD14基因启动子-260位点多态性。并分别采用生化方法和ELISA法检测研究对象的血脂和外周血sCD14水平。结果CD14启动子-260多态性位点基因型分布和等位基因频率在冠心病组和对照组间比较均无统计学差异(P均>0.05)。结论CD14基因启动子C(-260)T位点多态性与江苏地区汉族人群冠心病不存在相关性,它可能不是汉族人群冠心病发病的遗传学易感因素。
Objective To analyze the relationship between the C (-260) T polymorphism of CD14 gene and coronary heart disease in Han nationality in Jiangsu province. Methods PCR-RFLP was used to analyze the promoter-260 locus polymorphism of CD14 gene in 193 coronary artery disease patients diagnosed by coronary angiography and 225 healthy controls. Biochemical methods and ELISA were used to detect the level of sCD14 in serum and peripheral blood of the study subjects. Results There was no significant difference in genotype distribution and allele frequency between CD14 promoter - 260 polymorphism loci and control group (all P> 0.05). Conclusion There is no correlation between C (-260) T polymorphism of CD14 gene promoter and coronary heart disease in Han nationality in Jiangsu Province. It may not be a genetic susceptibility factor of coronary heart disease in Han population.