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目的研究围生期双酚A(BPA)暴露对♂子代大鼠海马CA1区神经元形态及突触可塑性的影响。方法对SD大鼠的母鼠从妊娠d11直至产后7d每日分别皮下注射3种剂量的BPA(10、100、1000μg·kg-1作为低、中、高剂量组),同时注射食用色拉油设立对照组。统计各组母鼠产仔数,并观察母鼠吃仔情况。在♂子鼠出生21d后,取脑组织切片,进行HE染色;或用电生理学方法检测BPA暴露对海马CA1区LTP及LTD诱导率的影响。结果高剂量BPA组母鼠吃仔率明显高于对照组(P<0.01);HE染色结果显示,高剂量BPA组♂子鼠海马CA1区锥体细胞发生核固缩变性,异常锥体神经元数量明显增多(P<0.05);低剂量BPA组♂子鼠海马脑片CA1区LTP诱导成功率与对照组相比明显降低(P<0.05),而LTD诱导成功率则明显升高(P<0.05)。结论围生期BPA暴露可以损伤♂子代大鼠海马CA1区神经元形态及突触传递可塑性。
Objective To investigate the effects of perinatal BPA exposure on neuronal morphology and synaptic plasticity in the hippocampal CA1 subfield of ♂ offspring. Methods Three doses of BPA (10, 100, 1000 μg · kg-1 as low, medium and high doses) were subcutaneously injected into dams of SD rats from d11 of gestation to 7 days of gestation, respectively. Simultaneously, edible salad oil was injected Control group. Statistics of each group litter size, and observation of euthanasia mothers. After 21 days of birth, ♂ mice were sacrificed and brain sections were harvested for HE staining. The effects of BPA exposure on the induction rate of LTP and LTD in hippocampal CA1 region were detected by electrophysiological methods. Results Compared with the control group, the rats in the high-dose BPA group had significantly higher eating-to-eat ratio (P <0.01). The results of HE staining showed that pyramidal neurons of hippocampal CA1 pyramidal neurons (P <0.05). The success rate of LTP induction in hippocampal CA1 subregion of the low dose BPA group was significantly lower than that of the control group (P <0.05), but the successful induction rate of LTD was significantly higher (P < 0.05). Conclusion Peripheral BPA exposure can impair neuronal morphology and synaptic transmission of hippocampal CA1 subfield in ♂ offspring rats.