目的:对现有的经腹部切口建立急性肾缺血再灌注损伤动物模型进行改良,探索建立急性肾缺血再灌注损伤模型的新方法。方法:实验组大鼠16例,经背部切口进入腹膜后间隙,游离钳夹双侧肾动脉45min后开放血流,建立急性肾缺血再灌注损伤模型;伪手术组8例,不夹闭肾动脉,余步骤与实验组相同;对照组8例无处理。术后通过建模成功率、组织病理检查、血肌酐和血尿素氮及氧化应激水平对模型进行评估。结果:实验组15只成功建立急性肾缺血再灌注损伤模型。术后1天病理检查显示实验组肾组织出现广泛损伤,术后实验组肾小管坏死评分、肾MDA水平、血肌酐及血尿素氮值明显高于对照组(P<0.05)。结论:经背部切口钳夹双侧肾动脉可建立稳定的大鼠急性肾缺血再灌注损伤模型。该造模方法简便易行,成功率高,且具备手术切口小、手术时间短及并发症少的优点,建立的模型适合于急性肾损伤的研究。 OBJECTIVE: To establish an animal model of acute renal ischemia-reperfusion injury through transabdominal incision and to explore a new method to establish a model of acute renal ischemia-reperfusion injury. Methods: Sixteen rats in the experimental group were injected into the retroperitoneal space through the dorsal incision, and the blood flow was opened after 45min free clamp of the bilateral renal arteries to establish a model of acute renal ischemia-reperfusion injury. In the sham operation group, 8 cases Arteries, the remaining steps and the same experimental group; control group of 8 patients without treatment. After the model success rate, histopathological examination, serum creatinine and blood urea nitrogen and oxidative stress levels were evaluated. Results: Fifteen rats in experimental group were successfully established acute renal ischemia-reperfusion injury model. One day after operation, the pathological examination showed extensive damage to the renal tissue in the experimental group. The postoperative tubule necrosis score, renal MDA level, serum creatinine and blood urea nitrogen were significantly higher than those in the control group (P <0.05). Conclusion: The model of acute renal ischemia-reperfusion injury in rats can be established by dorsal incision of bilateral renal arteries. The method has the advantages of simple and easy operation, high success rate, small operation incision, short operation time and few complications. The established model is suitable for the research of acute renal injury.