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目的:研究白杨素(ChR)是否具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。方法:体外培养SGC-7901细胞。MTT比色法测定细胞毒性。碘化丙啶(PI)染色流式细胞术(FCM)分析细胞死亡率。结果:ChR、rhsTRAIL以及两者合用对SGC-7901细胞活性的半数抑制浓度(IC50值)分别为134μmol/L、402ng/mL和47ng/mL,合并用药效应的CI值是0.4676。ChR40μmol/L、rhsTRAIL100ng/mL以及两者合用的细胞死亡率分别为4.65%±0.58%、3.60%±0.16%和49.87%±4.27%。结论:亚细胞毒性浓度的白杨素具有增强rhsTRAIL对人胃癌SGC-7901细胞毒性作用。
AIM: To investigate whether chrysin (ChR) can enhance the cytotoxicity of rhsTRAIL on human gastric cancer cell line SGC-7901. Methods: SGC-7901 cells were cultured in vitro. MTT assay for cytotoxicity. Propidium iodide (PI) staining flow cytometry (FCM) was used to analyze cell death rates. Results: The IC50 of ChR, rhsTRAIL and their combination on SGC-7901 cells were 134μmol / L, 402ng / mL and 47ng / mL, respectively. The CI value of combination therapy was 0.4676. Cell death rates of ChR40μmol / L, rhsTRAIL100ng / mL and their combination were 4.65% ± 0.58%, 3.60% ± 0.16% and 49.87% ± 4.27% respectively. CONCLUSION: Chrysin at sub-cytotoxic concentrations can enhance the cytotoxicity of rhsTRAIL on human gastric cancer cell line SGC-7901.