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目的研究滇重楼Paris polyphylla var.yunnanensis自然居群的遗传多样性,为滇重楼资源的保护和合理利用提供数据。方法采用SSR分子标记对滇重楼5个不同居群共115份样品进行遗传多样性分析。结果筛选出8对可用SSR引物,多态位点百分率(PPB)为100%,多态信息量(PIC)为0.745 6。在居群水平和物种水平上,观测等位基因数(N_a)分别为8.425 0和17.750 0,有效等位基因数(N_e)分别为4.960 9和7.500 7,观测杂合度(H_o)分别为0.295 5和0.294 8,期望杂合度(H_e)分别为0.654 8和0.774 4,Shannon’s信息指数(I)分别为1.520 1和2.038 6。遗传分化系数(F_(st))为0.172 8,基因流(N_m)为1.196 6。利用UPGMA聚类分析,将5个居群分为2类。结论滇重楼遗传多样性水平较高,居群内和居群间具有一定的遗传分化。对保护和发展滇重楼资源提出若干措施,为滇重楼的保护和科学可持续利用提供参考。
Objective To study the genetic diversity of natural populations of Paris polyphylla var. Yunnanensis and provide data for the protection and rational utilization of resources of this plant. Methods SSR markers were used to analyze the genetic diversity of 115 samples from 5 different populations of Yunnan reef. Eight pairs of SSR primers were screened out. The percentage of polymorphic loci (PPB) was 100% and the polymorphic information content (PIC) was 0.745 6. At population level and species level, the observed alleles (N_a) were 8.425 0 and 17.750 0 respectively, the number of effective alleles (N_e) was 4.960 9 and 7.500 7 respectively, and the observed heterozygosity (H_o) was 0.295 5 and 0.294 8 respectively. The expected heterozygosities (H_e) were 0.654 8 and 0.774 4, respectively. Shannon’s information index (I) was 1.520 1 and 2.038 6 respectively. The coefficient of genetic differentiation (F st) was 0.172 8, and the gene flow (N_m) was 1.196 6. Using UPGMA cluster analysis, five populations were divided into two categories. Conclusion The level of genetic diversity in Yunnan Zhonglou is high, with a certain degree of genetic differentiation within and between populations. Several measures are put forward for the protection and development of the resources of Yunnan Zhonglou, so as to provide reference for the protection and sustainable use of science in Yunnan.