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目的:对中国恒河猴感染猴免疫缺陷病毒(SIV)后CD4T初始、记忆亚群的细胞进行分析,了解各亚群的变化规律以及在AIDS发病过程中的意义。方法:使用SIVmac239毒株感染20只中国恒河猴,观察18个月;在感染前、后各时间点,使用实时荧光定量PCR(TaqMan探针法)进行病毒载量的检测;使用CD3/CD4/CD28/CD95表面标记的组合,进行CD4及其初始/记忆亚群比例的流式分析,并结合血常规检测的结果计算CD4及其各亚群的绝对数。结果:得到了CD4各个亚群在SIV感染后的变化曲线;并发现SIV感染后CD4减少的主体是中央型记忆(CM)CD4+T细胞;而初始型CD4+T在半数动物逐渐缓慢减少,但个体间差异较大。此外,还发现快速进展型RM449猴在死亡前其初始型及CM CD4仍较高,但其初始型CD4细胞在流式图上存在着类似“转化阻滞”的现象。结论:本研究展示了CD4细胞的初始、记忆亚群在感染SIV后的变化规律;对其进行细分研究,有助于更准确地了解体内免疫状态的变化,为临床疗效观察、免疫状态评估等提供参考。
OBJECTIVE: To analyze the CD4 (+) and memory (MEM) subpopulations of rhesus monkeys infected with simian immunodeficiency virus (SIV) and to understand the changes of subpopulations and their significance in the pathogenesis of AIDS. Methods: Twenty Chinese rhesus monkeys were infected with SIVmac239 strain and observed for 18 months. The viral load was detected by real-time fluorescence quantitative PCR (TaqMan probe method) before and after infection. CD3 / CD4 / CD28 / CD95 surface markers, CD4 and its initial / memory subsets were analyzed by flow cytometry and the absolute numbers of CD4 and its subsets were calculated in combination with the results of routine blood tests. Results: The changes of CD4 subtypes after SIV infection were obtained. It was also found that the main body of CD4 depletion after SIV infection was central memory (CM) CD4 + T cells. The initial CD4 + T gradually decreased in half of the animals, But the difference between individuals is larger. In addition, rapid development of RM449 monkey was found before its death, its initial type and CMCD is still high, but its initial type CD4 cells in the flow chart there is a similar “conversion block ” phenomenon. CONCLUSIONS: This study demonstrates the changing pattern of the initial, memory subsets of CD4 cells after infection with SIV. Subdivision studies may help to understand more accurately the changes in the immune status in vivo, and provide evidence for clinical efficacy, assessment of immune status For reference.