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目的探讨刺槐素在不同时间点给药对小鼠脑缺血再灌注损伤的保护作用,并分析刺槐素的治疗时间窗。方法将36只雄性昆明小鼠随机分为假手术组、缺血再灌注组、刺槐素(治疗)组,治疗组又根据不同给药时间分为0、2、4、8h 4个给药组,每组各6只;采用线栓法制作大脑中动脉闭塞模型,治疗组予缺血0、2、4、8h腹腔注射刺槐素(12.5 mg/kg);再灌注后24h进行神经功能评分,应用2,3,5-氯化三苯基四氮唑染色测定脑梗死体积。结果缺血0、2、4h给药组神经功能评分均显著低于缺血再灌注组(p均<0.05),8h给药组神经功能评分有改善,但无统计学意义(p=0.052);不同时间给药组间神经功能评分无统计学意义(p>0.5)。缺血再灌注组脑梗死体积为(65.18±5.96)mm~3,与缺血再灌注组相比,缺血0h[(22.15±3.02)mm~3,p=0.000],2h[(28.61±4.19)mm~3,p=0.000],4h[(49.8±4.08)mm~3,p=0.000]给药组脑梗死体积均显著降低,8h[(56.09±7.92)mm~3,p=0.075]给药组脑梗死体积有减小,但无统计学意义。结论在缺血早期应用刺槐素治疗可有效改善神经功能评分,缩小脑梗死体积。
Objective To investigate the protective effect of acaciacinol on cerebral ischemia-reperfusion injury in mice at different time points and to analyze the treatment time window of acacin. Methods Thirty-six male Kunming mice were randomly divided into 4 groups: sham-operation group, ischemia-reperfusion group and acaciacin treatment group. The treatment group was divided into 4 groups: 0, 2, (6 rats in each group). Middle cerebral artery occlusion (MCAO) model was made by thread occlusion. The rats in the treatment group were given intraperitoneal injection of acrolein (12.5 mg / kg) at 0, 2, 4 and 8 hours after ischemia, Cerebral infarction volume was measured by 2,3,5-triphenyltetrazolium chloride staining. Results The scores of neurological function in the 0,2,4 h ischemia group were significantly lower than those in the ischemia / reperfusion group (all p <0.05), while the neurological function scores in the 8h group were improved, but not statistically significant (p = 0.052) There was no significant difference in neurological function between the two groups (p> 0.5). The volume of cerebral infarction in ischemia / reperfusion group was (65.18 ± 5.96) mm ~ 3, and compared with ischemia reperfusion group, the infarct volume in 0h [(22.15 ± 3.02) mm ~ (49.09 ± 7.98) mm ~ 3, p = 0.000]. The volume of cerebral infarction in the treatment group was significantly lower than that in the control group The volume of cerebral infarction in the treatment group was decreased, but not statistically significant. Conclusion Application of acacia in the early stage of ischemia can effectively improve the score of neurological function and reduce the volume of cerebral infarction.