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目的了解慢性前列腺炎与病原体的关系,探讨前列腺按摩液(EPS)中病原体的临床意义。方法常规按摩法获取患者EPS,进行湿涂片常规镜检白细胞(WBC)和卵磷脂小体;普通细菌培养直接接种血琼脂平皿,淋病奈瑟菌用巧克力琼脂培养,衣原体用单克隆抗体胶体金标法,支原体用尿素酶颜色试验培养液法,药物敏感性试验用K-B琼脂扩散法。结果480例患者前列腺液常规检查WBC>10/HP,卵磷脂颗粒均有不同程度的减少。普通细菌培养共分离出31株细菌,分离率为6.5%。其中球菌17株,占54.8%;杆菌11株,占35.5%;真菌3株,占9.6%。细菌种类以葡萄球菌、大肠埃希菌和甲型溶血性链球菌为主;特殊培养中共分离出病原体109株,分离率为22.7%。其中沙眼衣原体、支原体、淋球菌分别为71株、22株、16株。结论EPS中病原体检测对临床诊断和治疗慢性前列腺炎具有重要意义。
Objective To understand the relationship between chronic prostatitis and pathogens and to explore the clinical significance of pathogens in prostate massage fluid (EPS). Methods Conventional massage method was used to obtain the EPS of patients and routine smear white blood cells (WBC) and lecithin bodies on wet smears. The common bacterial culture was directly inoculated on blood agar plates, while Neisseria gonorrhoeae was cultured on chocolate agar. Chlamydia trachomatis with monoclonal antibody colloidal gold Standard method, Mycoplasma urease color test medium method, drug susceptibility testing using KB agar diffusion method. Results 480 cases of prostatic fluid routine examination WBC> 10 / HP, lecithin particles were reduced to varying degrees. A total of 31 strains of bacteria were isolated from common bacterial culture, with a separation rate of 6.5%. Including cocci 17, accounting for 54.8%; 11 strains of bacteria, accounting for 35.5%; 3 strains of fungi, accounting for 9.6%. Staphylococcus aureus, Escherichia coli and Streptococcus hemolyticus were the main bacterial species. 109 strains of pathogens were isolated from the special culture, the isolation rate was 22.7%. Among them, Chlamydia trachomatis, Mycoplasma and Neisseria gonorrhoeae were 71, 22 and 16, respectively. Conclusion The detection of pathogens in EPS is of great significance in clinical diagnosis and treatment of chronic prostatitis.