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旨在探讨叶酸对炔雌醚致雄性大鼠生殖损伤的保护作用。将20只8周龄成年雄性SD大鼠适应饲养1周后,随机分为4组。对照组:橄榄油+生理盐水;炔雌醚组:1.0mg·kg-1炔雌醚;炔雌醚+叶酸组:1.0mg·kg-1炔雌醚+1.1mg·kg-1叶酸;叶酸组:1.1mg·kg-1叶酸;灌胃处理,每天一次,连续2周。处理结束后,取生殖器官称重,检测精子质量,测定睾丸抗氧化功能,制作睾丸组织切片,观察睾丸组织结构,通过免疫组织化学染色检测Caspase-3与eNOS表达。结果显示,炔雌醚单独处理组造成大鼠生殖器官萎缩,重量下降,精子发生异常,精子质量下降,抗氧化酶活性下降,睾丸发生氧化应激;与之相反,凋亡效应酶Caspase-3与eNOS表达增加。炔雌醚+叶酸处理组通过减少eNOS表达降低氧化应激,抑制Caspase-3表达,降低精子畸形率、生精小管损伤,最终改善雄性大鼠生殖功能。与对照组相比,单独叶酸处理组睾丸抗氧化酶活性、组织结构和精子质量均有一定程度提高,无显著差异。本研究首次证实叶酸有效地抑制了炔雌醚对雄性大鼠生殖功能的破坏作用。
To explore the folic acid on ethinylestradiol induced reproductive injury in male rats. Twenty adult male SD rats, 8 weeks old, were housed for 1 week, then randomly divided into 4 groups. Control group: olive oil + saline; ethinyl estradiol group: 1.0 mg · kg -1 ethynylestradiol; ethynylestradiol + folic acid group: 1.0 mg · kg -1 ethinyl equolexate + 1.1 mg · kg -1 folic acid; folic acid Group: 1.1mg · kg-1 folic acid; intragastric treatment once a day for 2 weeks. After the treatment, the reproductive organs were weighed, the sperm quality was measured, the antioxidant function of the testis was measured, the testis tissue sections were made, the testis tissue structure was observed, and the expressions of Caspase-3 and eNOS were detected by immunohistochemical staining. The results showed that alkyne estrogen alone treatment group caused rat atrophy of the reproductive organs, weight loss, sperm abnormalities, sperm quality decline, antioxidant enzyme activity decreased testicular oxidative stress; In contrast, the apoptotic effect of the enzyme Caspase-3 And eNOS expression increased. Alveolar estrone + folic acid treatment group decreased oxidative stress, inhibited Caspase-3 expression, decreased sperm deformity rate, induced seminiferous tubule damage, and ultimately improved reproductive function of male rats by reducing eNOS expression. Compared with the control group, the antioxidant enzyme activity, tissue structure and sperm quality of testis in folic acid alone group increased to a certain extent, with no significant difference. This study demonstrated for the first time that folic acid effectively inhibits the damaging effects of ethinyl estradiol on reproductive function in male rats.