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姊妹染色单体互换(SCE)是指两条姊妹染色单体在相同位置上发生的同等对称的片段交换。目前显示SCE的方法除荧光染料法(Latt),荧光加Giemsa法(FPG法)外,尚有一些改良方法如:0.1%碱性品红直接染色法,0.3mol/LNa_2HPO_4-Giemsa法,4Na-EDTA-Giemsa法等,这些方法都适用于SCE频率的检出。但用以往的方法拟进一步对SCE位点进行定位时,需将片子重新脱色,显带才能完成;而且脱色后显带效果不稳定,带型不清,方法复杂并且定位有时并不十分精确。本文介绍了一种在染色体标本上一次同时显示SCE及G带带型的新方法。此法过程简便,结果稳定,定位准确,弥补了以往方法的不足,是研究SCE位点在染色体各区带之间分布的一个有效手段。
Sister chromatid exchange (SCE) refers to the same symmetrical segment exchange occurs in the same position of two sister chromatids. At present, there are some improved methods such as the method of 0.1% alkaline fuchsin, the method of 0.3mol / LNa_2HPO_4-Giemsa, the method of 4Na- EDTA-Giemsa method, these methods are suitable for the detection of SCE frequency. However, when using the conventional method to further locate the SCE site, the film needs to be decolorized again, and the banding can be completed. Moreover, the banding effect after destaining is unstable, the banding type is unclear, the method is complicated and the positioning is sometimes not very accurate. This article presents a new method for simultaneously displaying SCE and G-banding patterns on a chromosome specimen. The method has the advantages of simple and convenient procedure, stable result and accurate positioning, which can make up for the deficiency of previous methods and is an effective method for studying the distribution of SCE loci among the chromosomes.