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目的探讨人类白细胞抗原(HLA)-DRB1等位基因多态性与新生儿溶血病(HDN)发生之间的关系。方法对117例HDN患者和112例非新生儿溶血病(NHDN)标本,采用PCR-SSO方法进行HLA-DRB1基因分型,对基因频率以及疾病发生的相对危险度(RR)进行研究。结果 HDN患者中HLA-DRB1*1202的基因频数较NHDN显著升高(χ2=7.653,P<0.01,RR=2.598);含有HLA-DRB1*1202基因位点的HDN患者,其总胆红素值升高较不含该基因的患者差异有统计学意义(χ2=22.582,P<0.01)。结论 HLA-DRB1*1202与HDN的易感性呈正相关,可能是其易感基因。
Objective To investigate the relationship between human leukocyte antigen (HLA) -DRB1 allele and neonatal hemolytic disease (HDN). Methods A total of 117 patients with HDN and 112 non-neonatal hemolytic disease (NHDN) specimens were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-SSO). The frequency of the gene and the relative risk (RR) of the disease were studied. Results The frequency of HLA-DRB1 * 1202 gene in patients with HDN was significantly higher than that in NHDN patients (χ2 = 7.653, P <0.01, RR = 2.598). The HDN patients with HLA-DRB1 * 1202 locus had higher total bilirubin The difference was statistically significant (χ2 = 22.582, P <0.01). Conclusion HLA-DRB1 * 1202 is positively correlated with the susceptibility of HDN and may be the predisposing gene.