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西藏林芝地区冬虫夏草天然产区野生虫草蝙蝠蛾幼虫的肠道液p H范围为9.69–10.99,体外在此p H下培养冬虫夏草菌长势较差。同时,在该p H的体外模拟中肠液中,虫菌体也会在极短的时间内消亡,而对自然侵染的蝙蝠蛾幼虫的血淋巴、消化道及其“反吐物”(肠液)进行切片和显微镜检却发现,蝙蝠蛾幼虫消化道、呕吐物中均存在与血淋巴中相同的冬虫夏草菌菌体形态,生存状态良好,正常增殖,表明蝙蝠蛾幼虫肠道的生理状态可以维系并支撑冬虫夏草菌菌体某种形态的存在与生长,并证实冬虫夏草菌由口器及消化道入侵蝙蝠蛾幼虫的可能性;肠道虫菌体与血淋巴虫菌体不一定同时存在,表明冬虫夏草菌对蝙蝠蛾幼虫的侵染可能同时存在表皮侵染和肠道侵染两种途径。本文为冬虫夏草菌对蝙蝠蛾幼虫的侵染途径研究提供更加多样化的信息支持。
The intestinal fluid p H range of the wild C. sinensis larvae in the native Cordyceps sinensis natural area in Linzhi Prefecture of Tibet was 9.69-10.99. The growth of Cordyceps sinensis in this p H in vitro was poor. At the same time, the mycobacteria also die in a very short period of time in the in vitro simulated mid-intestinal fluid of p H, whereas the naturally infected bat moth larvae hemolymph, digestive tract and their “anti-spit” Intestinal juice) section and microscopic examination found that the bat moth larvae digestive tract, vomit and hemolymph are present in the same Cordyceps bacteria morphology, good survival, normal proliferation, that the bat moth larvae of the physiological state of the intestine can Maintain and support the existence and growth of a certain morphology of Cordyceps sinensis bacteria and confirm the possibility that Cordyceps sinensis will invade the bat moth larvae by the mouthparts and digestive tract; the intestinal bacteria and hemolymph bacteria do not necessarily exist at the same time, indicating Cordyceps sinensis infection of bat moth larvae may exist both epidermal and intestinal infection in two ways. This article provides more diversified information support for the study on the infection pathways of Cordyceps sinensis to bat moth larvae.